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Coding
Intein

Part:BBa_K2075002

Designed by: Louise Schaar   Group: iGEM16_Hannover   (2016-10-13)


Intein

Inteins were integrated into proteins. They useful by the circularization

of a protein by ligating the N- and C-terminal ends. The team Heidelberg 2014 already used them for thei r circulation but we changed the sequence to make the circularization more accurate. They successful showed that circularization enhances resistance against proteases and denaturation. We show t hat circularization with a TAL-effector makes them more solid and durable for everyday laboratory.

If you want more Information have a look of our results. The intein sequence have a size of 309 bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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